Build_PGAdb Build_wgMLSTtree   Build_PGAdb
output sample
Build_wgMLSTtree output sample Help
 

PGAdb-builder User Guide



Step 1: Build PGA Database

Build_PGAdb input: upload genome contig files



Build_PGAdb output: graphical output example and explanation


The above image is an example of the graphical output of the Build_PGAdb.

Content of the Build_PGAdb output

  • A summary of the setting used in the execution
  • A summary of output files that users can download
  • A pie chart shows the composition of the pan genome database
  • A continue execution button for Build_wgMLSTtree



Step 2: Build wgMLST Tree

Build_wgMLSTtree input: upload genome contig files


Database ID:

  • User can use their own PGAdb for Build_wgMLSTtree by inputing the unique "Database ID".

Coverage & Identity:

  • The parameters define the thresholds of "coverage" and the "identity" when blasting the uploaded genome contigs to the PGAdb.

Schemes:

  • The "loci set" for Build_wgMLSTtree.

Build_wgMLSTtree output: graphical output example and explanation


The above image is an example of the graphical output of the Build_wgMLSTtree.

Content of the Build_wgMLSTtree output

  • A summary of the setting used in the execution
  • A summary of output files that users can download
  • A execution button for regenerating the genetic relatedness tree


 
 
Copyright © 2016 Centers for Disease Control, Taiwan (Taiwan CDC) and Institute of Medical Science and Technology, National Sun Yat-sen University, Taiwan. Contact
Liu YY, Chiou CS & Chen CC. PGAdb-builder: A web service tool for creating pan-genome allele database for molecular fine typing. Sci. Rep. 6, 36213; doi: 10.1038/srep36213 (2016).